<i>Legionella pneumophila</i> modulates the host cytoskeleton by an effector of transglutaminase activity.

ABSTRACT: The bacterial pathogen Legionella pneumophila delivers more than 330 effector proteins into host cells through its Dot/Icm type IV secretion system (T4SS) to facilitate its intracellular replication. A number of these effectors modulate organelle trafficking pathways to create a membrane-bound niche called the Legionella-containing vacuole (LCV). In this study, we found that L. pneumophila induces F-actin accumulation in the host cell cortex by its Dot/Icm substrate RavJ (Lpg0944). RavJ harbors a C₁₀₁H₁₃₈D₁₇₀ motif associated with human tissue transglutaminases (TGs). We show that RavJ catalyzes a covalent linkage between actin and members of the Motin family of proteins, including Angiomotin (AMOT) and Angiomotin-like 1 (AMOTL1), which

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12207909 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature