SNACS: a tool for demultiplexing single-cell DNA sequencing data.

ABSTRACT:

Motivation

Single-cell DNA sequencing (scDNA-seq) and multi-modal profiling with the addition of cell-surface antibodies (scDAb-seq) have recently provided key insights into cancer heterogeneity. Scaling these technologies across large patient cohorts, however, is cost and time prohibitive. Multiplexing, in which cells from unique patients are pooled into a single experiment, offers a possible solution. While multiplexing methods exist for scRNAseq, accurate demultiplexing in scDNAseq remains an unmet need.

Results

Here, we introduce SNACS: single-nucleotide polymorphism and antibody-based cell sorting. SNACS relies on a combination of patient-level cell-surface identifiers and natural variation in genetic polymorphisms to demultiplex scDNAseq data. We demonstrated the perfo

SUBMITTER: Kennedy VE 

PROVIDER: S-EPMC12208073 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature