Genome characterisation of the first isolate of human enterovirus c99 from an acute flaccid paralysis case in Brazil.

ABSTRACT:

Background

Human enterovirus C99 (HEV-C99) is a member of the species Enterovirus C. Currently, three complete genomes of HEV-C99 were reported in Brazil, all obtained from children with gastroenteritis symptoms. Notwithstanding, no HEV-C99 complete genome associated with AFP cases in Brazil have been analysed so far.

Objectives

In light of this, molecular characterisation of an HEV-C99 isolated from a case of acute flaccid paralysis (AFP) in Brazil was carried out.

Methods

In 2005, an HEV-C99 strain was isolated from a 2-year-old female child in Santa Catarina State, Brazil, showing classic symptoms of AFP. Stool sample was inoculated into specific cell cultures. Viral RNA was extracted, and polymerase chain reaction (PCR) were performed to amplify the VP1 gene; the

SUBMITTER: Sauthier JT 

PROVIDER: S-EPMC12208675 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature