Whole-genome sequencing and biofilm gene characterization in multidrug-Resistant Staphylococcus aureus clinical strains.

ABSTRACT: Staphylococcus aureus is known as a significant contributor to a variety of severe, life-threatening illnesses. Infectious diseases associated with biofilm-producing S. aureus can lead to a substantial increase in morbidity and mortality rates. This study aimed to characterize the whole genomes of eight clinically multidrug-resistant S. aureus strains isolated from several types of human infections sites from Hail Hospital, Saudi Arabia. Biofilm production was evaluated using Congo-red agar plates (CRA), polystyrene microtiter plate technique (MtP), and adherence to human epithelial cells (Hep 2). Additionally, adhesion to abiotic surface (Polyethylene, glass, stainless steel) was assessed using scanning electron microscopy (SEM). Then whole genome sequencing was conducted for all strains

SUBMITTER: Noumi E 

PROVIDER: S-EPMC12208944 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature