Poly(ADP-ribose)polymerase 2 is zinc-dependent enzyme and nucleosome reorganizer.

ABSTRACT: Poly(ADP-ribose)polymerase 2 (PARP2) is a nuclear protein, DNA damage sensor and an emerging target for development of anti-cancer drugs. Previously it was discovered that PARP2 binds to nucleosomes; however, critical factors involved in this process remain unknown. We demonstrated that in the presence of Mg²⁺ or Ca²⁺ ions PARP2 forms complexes with a nucleosome containing different number of PARP2 molecules without altering conformation of nucleosomal DNA. In contrast, Zn²⁺ ions directly interact with PARP2 inducing a local alteration of the secondary structure of the protein and PARP2-mediated, reversible structural reorganization of nucleosomes. WGR domain of PARP2 is the target for Zn²⁺ ions since this domain contains two putative Zn^2+-<

SUBMITTER: Maluchenko N 

PROVIDER: S-EPMC12209172 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature