Electroacupuncture Attenuates High-Fat Diet-Exacerbated Alzheimer's Pathology by Enhancing TFEB/TFE3-Mediated Autophagic Clearance of Tau and NLRP3 Inflammasome in 3xTg Mice.

ABSTRACT:

Background

Alzheimer's disease (AD) pathology is increasingly linked to metabolic disturbances induced by high-fat diets (HFD), with both autophagy-lysosomal pathway (ALP) dysfunction and activation of the NLRP3 inflammasome emerging as key pathological mechanisms. This study introduces a novel approach using electroacupuncture (EA) to mitigate HFD-accelerated AD pathology, focusing on the regulatory role of transcription factors TFEB and TFE3 in ALP and their potential modulation by EA.

Methods

3xTg-AD mice were fed either an HFD or a standard diet for 16 weeks, followed by EA treatment at the ST36 acupoint. AAV-mediated hippocampal knockdown of TFEB and TFE3 was performed using shRNA constructs. Cognitive performance was evaluated with the Morris water maze (MWM) and Y-maz

SUBMITTER: Zheng X 

PROVIDER: S-EPMC12209595 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature