Antibiotic stress affects the secretion and physicochemical features of extracellular vesicles produced by Helicobacter pylori.

ABSTRACT:

Background

Bacterial extracellular vesicles (EVs) may reduce the effectiveness of various antimicrobials; however, the impact of antibiotics on the secretion and properties of EVs produced by Helicobacter pylori has not been established.

Methods

Using clinical H. pylori strains and culture in EV-depleted media, the influence of ¼ × MIC of clarithromycin, metronidazole and levofloxacin on EV features was determined. Physicochemical properties of EVs were measured using nanoparticle tracking analysis and dynamic light scattering. Determination of fatty acid profiles of EVs and bacterial cells was performed with GC triple-quadrupole tandem MS. Bacteria and EVs were observed by scanning and transmission electron microscopy, respectively.

Results

Antibiotic stress induced

SUBMITTER: Krzyzek P 

PROVIDER: S-EPMC12209803 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature