Segmented milli-fluidic crystallisation of paracetamol with <i>in situ</i> single-crystal X-ray diffraction.

ABSTRACT: Serial crystallography has revolutionalised our ability to analyse protein crystals; crystal structures can be uncovered by combining data from multiple crystals mitigating radiation damage through overexposure to the X-ray beam. With synchrotron sources becoming even brighter, radiation damage is ever more pertinent for small molecule crystals as well. Combining serial crystallography with flow crystallisation, we pave the way for exploring high-throughput screening and kinetic studies, with application to small molecule crystals up to mm-scale. Here we present the first known example of single crystal X-ray diffraction of small molecule crystals in a flow crystallisation environment. In situ single-crystal X-ray diffraction has been achieved on a series of growing singular crystal

SUBMITTER: Wayment LE 

PROVIDER: S-EPMC12210179 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature