Functional and structural characterization of Stenotrophomonas maltophilia EntB, an unusual form of isochorismatase for siderophore synthesis.

ABSTRACT: Clinical and environmental isolates of Stenotrophomonas maltophilia produce an enterobactin-like siderophore that promotes bacterial growth under low-iron conditions. Although prior mutational and bioinformatic analyses indicated that most of the enzymes encoded by the S. maltophilia entCEBB'FA locus are suitably reminiscent of their counterparts in Escherichia coli and other bacteria, Stenotrophomonas EntB was unusual. In bacteria producing enterobactin-related molecules, EntB and its homologs are usually multi-domain proteins in which the amino portion acts as an isochorismatase and the carboxy domain serves as an aryl carrier protein (ArCP). However, in S. maltophilia the isochorismatase and ArCP functions are encoded by two distinct genes: entB and entB', respectively. Current mutant a

SUBMITTER: Nas MY 

PROVIDER: S-EPMC12210187 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature