Eliminating the Interference of Neighboring Nucleobases in Aerolysin for Nanopore Sequencing.

ABSTRACT: Biological nanopores have revolutionized DNA sequencing with their incredible advantages of long reads, high throughput, portability, and low material requirement. Despite numerous improvements, base calling remains challenging due to the influence of neighboring nucleobases at the reading site. One direction of development is exploring or designing new nanopores to improve base calling accuracy. Aerolysin is emerging as a powerful candidate, which can identify the subtle differences of detected molecules. However, not many studies focus on translating these advantages into DNA sequencing. Here, we used streptavidin to immobilize ssDNA inside an engineered aerolysin channel, which allows us to investigate its sensing regions, and the neighboring bases influence. By increasing the voltage,

SUBMITTER: Camazzola A 

PROVIDER: S-EPMC12210254 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature