Fecal microbiome profiling of children with <i>Shigella</i> diarrhea from low- and middle-income countries.

ABSTRACT: Shigella is one of the leading pathogens causing diarrhea in children globally. Stool culture remains the standard for diagnosing Shigella infections; however, quantitative PCR (qPCR) offers greater sensitivity. In this study, we evaluated the fecal microbiomes of 27 diarrheal children with and without Shigella identified by culture and quantitative PCR, respectively, aiming to characterize the fecal microbiome profiles of children with Shigella diarrhea and to explore the differences in microbiome and the mechanisms associated with the culturability of Shigella. Our results showed that Shigella qPCR positive cases were characterized by a significantly higher abundance of Shigella and other Proteobacteria, alongside a lower abundance

SUBMITTER: Fan Y 

PROVIDER: S-EPMC12211052 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature