A dual-excitation ratiometric NIR-II fluorescent nanoplatform enables high contrast <i>in vivo</i> imaging.

ABSTRACT: Ratiometric fluorescent probes in the second near-infrared window (NIR-II) with a self-calibration function are sought after for reliable imaging of physiological and pathological processes. Nevertheless, current ratiometric NIR-II fluorescent probes usually show severe spectral overlap in the emission channel, resulting in inevitable sacrifice of the emission intensity of the probe and compromised imaging quality in the NIR-II region. To address these challenges, we developed a novel dual-excitation ratiometric NIR-II fluorescence nanoplatform (DERF-NP), in which the intensity ratio of the same full-wavelength emission from 1000 to 1700 nm under two non-overlapping monochrome excitations with distinct responses is conventionally defined as the quantification parameter. As a proof of conce

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12211233 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature