Identification of the pyroptosis, apoptosis, and necroptosis (PANoptosis) involved in osteogenic differentiation inhibition impaired by tumor necrosis factor-α.

ABSTRACT:

Background

Inflammatory diseases of the bones often result in excessive release of inflammatory cytokines and impaired osteogenic differentiation. Tumor necrosis factor-α (TNF-α) is an important inflammatory cytokine in disease, and implicated in developing PANoptosis. Although TNF-α has captured much attention in PANoptosis, their relationship in osteogenic differentiation remains elusive. Therefore, it is necessary to explore how TNF-α inhibits osteogenic differentiation and the mechanism involved.

Methods

RNA sequencing was used to sequence normal and TNF-α-treated cells and screen for differentially expressed 43 genes of PANotosis. PCR and Western blotting were performed to validate sequencing results consistent. The degree of osteoblast differentiation was determined by

SUBMITTER: Xia CL 

PROVIDER: S-EPMC12211273 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature