Migration of Kupffer's vesicle-derived cells is essential for tail morphogenesis in zebrafish embryos.

ABSTRACT: A phenotypic screen of fungal filtrates on developing zebrafish embryos identified metabolites from the fungus Ceratocystis populicola to induce ectopic tail formation, including a split notochord and a duplicated caudal fin. Chemical analyses led to the identification of monoterpene alcohols, in particular geraniol, as the active compounds. Tüpfel long fin zebrafish embryos were more susceptible to geraniol-induced ectopic tail formation than Wild Indian Karyotpe zebrafish embryos. RNA-sequencing on tail buds of 15-somite-stage embryos revealed downregulation of essential genes of the retinoic acid signaling pathway. Differential expression of cyp26a1, fgf8a and downstream hox-genes was validated. Time-lapse imaging revealed that Kupffer's vesicle-derived cells failed to migrate after Kup

SUBMITTER: Hoeksma J 

PROVIDER: S-EPMC12211557 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature