Development of shotgun metabolomic profile analysis for detecting canine visceral leishmaniasis using flow-through pinhole paper spray mass spectrometry.

ABSTRACT: Visceral leishmaniasis (VL) is a neglected infectious disease that can be transmitted between dogs and humans. In this work, a novel flow-through pinhole paper spray mass spectrometry (MS) technique is described to detect metabolites that might be up- or down-regulated during canine VL infection. The flow-through pinhole paper spray MS allows direct analysis of dried samples prepared in paper substrates, without prior sample pre-treatment. Here, clinical canine serum samples from ten dogs positives for VL were prepared in embossed hydrophobic paper substrates in the lab. Similar dried serum samples from uninfected control dogs were prepared in embossed paper. Partial least squares discriminant analysis was performed on positive-ion mode full mass spectra recorded from the 20

SUBMITTER: Akuffu ED 

PROVIDER: S-EPMC12211625 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature