Clinical significance and biological function of PRKCQ-AS1/miR-582-3p expression in LUAD.

ABSTRACT:

Objective

To investigate the clinical value and mechanism of action of long non-coding RNA PRKCQ-AS1 for lung adenocarcinoma (LUAD) progression.

Methods

Clinical data of 128 LUAD patients were collected, postoperative pathological tissues were stored at -80 °C. Kaplan-Meier survival analysis was employed to investigate differences in 5-year survival rates across various expression groups, while Cox regression models assessed the prognostic factors influencing patient outcomes. Reverse transcription quantitative PCR (RT-qPCR) was utilized to measure the expression levels of PRKCQ-AS1 and miR-582-3p in pathological tissues and LUAD cell lines. Additionally, a dual-luciferase reporter assay validated the reciprocal relationship. CCK8 examined cell proliferation, Transwell obser

SUBMITTER: Liu L 

PROVIDER: S-EPMC12211715 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature