Construction and evaluation of recombinant Lactobacillus plantarum expressing Micropterus salmoides hepcidin.

ABSTRACT:

Background

Hepcidin is an antimicrobial peptide involved in innate immunity, and the addition of hepcidin or probiotics expressing hepcidin to feed can significantly enhance the disease resistance of farmed animals. Lactobacillus plantarum has the advantages of stable colonization in the animal gut, promotion of intestinal health, and efficient expression of foreign proteins. Previously, we have used L. plantarum (LP37) as a feed additive to promote the growth of Micropterus salmoides and Oreochromis mossambicus. The aim of this study is to construct a recombinant LP37 expressing hepcidin and evaluate its role in improving antibacterial ability and growth promotion in M. salmoides.

Results

Based on the expression vector pSIP403, we used green fluorescent protein (GFP) as a r

SUBMITTER: Zhang CL 

PROVIDER: S-EPMC12211718 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature