BRD4 acts as a transcriptional repressor of RhoB to inhibit terminal erythropoiesis.

ABSTRACT:

Background

Terminal erythropoiesis is a complex multistep process involving coordination of gene transcription and dramatic nuclear condensation, which leads to the expulsion of nuclei to generate reticulocytes. However, we lack a comprehensive understanding of the key transcriptional and epigenetic regulators involved.

Methods

We used a high-throughput small molecule screen in primary CD34⁺-derived human erythroblasts to identify targets that promoted terminal erythropoiesis, and further confirmed the phenotype in different differentiation systems by inhibitors and shRNAs of different BRD4 isoforms. Then we performed RNA-seq, ATAC-seq, ChIP-qPCR, Co-IP, and reanalyzed previously-published transcriptional data and mass spectrometric data to clarify how BRD4 regula

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12211804 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature