Tick genomics through a Nanopore: a low-cost approach for tick genomics.

ABSTRACT:

Background

The assembly of large and complex genomes can be costly since it typically requires the utilization of multiple sequencing technologies and access to high-performance computing, while creating a dependency on external service providers. The aim of this study was to independently generate draft genomes for the cattle ticks Rhipicephalus microplus and R. appendiculatus using Oxford Nanopore sequencing technology.

Results

Exclusively, Oxford Nanopore sequence data were assembled with Shasta and finalized on the Amazon Web Services cloud platform, capitalizing on the availability of up to 90% discounted Spot instances. The assembled and polished R. microplus and R. appendiculatus genomes from our study were comparable to published tick genomes where multiple sequencin

SUBMITTER: Meiring C 

PROVIDER: S-EPMC12211944 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature