High throughput screening for the design of protein binding polymers.

ABSTRACT: Using polymers for protein encapsulation can enhance stability in processing environments and prolong activity and half-life in vivo. However, finding the best polymer structure for a target protein can be difficult, labour- and cost-intensive. In this study we introduce a high throughput screening approach to identify strong polymer-protein interactions by use of Förster Resonance Energy Transfer (FRET), enabling a rapid read out. We iteratively screened a total of 288 polymers containing varying hydrophilic, hydrophobic, anionic and cationic monomers against a panel of eight different enzymes (glucose oxidase, uricase, manganese peroxidase, bovine serum albumin, carbonic anhydrase, lysozyme, trypsin and casein). By optimisation of the assay conditions it was possible to read out s

SUBMITTER: Bapp C 

PROVIDER: S-EPMC12212005 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature