Tissular chromatin-state cartography based on double-barcoded DNA arrays that capture unloaded PA-Tn5 transposase.

ABSTRACT: Recent developments in spatial omics are revolutionizing our understanding of tissue structure organization and their deregulation in disease. Here, we present a strategy for capturing chromatin histone modification signatures across tissue sections by taking advantage of a double-barcoded DNA arrays design compatible with in situ Protein A-transposase Tn5 tagmentation. This approach has been validated in presence of fresh-frozen mouse brain tissues but also in decalcified formalin-fixed paraffin-embedded (FFPE) mouse paw samples, in which either the histone modification H3K4 trimethylation or H3K27 acetylation has been used as proxy for interrogating active promoter signatures. Furthermore, because combinatorial enrichment of multiple histone modifications was shown to code for various st

SUBMITTER: Mendoza-Ferri MG 

PROVIDER: S-EPMC12212351 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature