Integrated single-cell multiome analysis reveals muscle fiber-type gene regulatory circuitry modulated by endurance exercise.

ABSTRACT: Endurance exercise induces multisystem adaptations that improve performance and benefit health. Gene regulatory circuit responses within individual skeletal muscle cell types, which are key mediators of exercise effects, have not been studied. Here, we map transcriptome, chromatin, and regulatory circuit responses to acute endurance exercise in muscle using same-cell RNA-seq/ATAC-seq multiome assays. High-quality data were obtained from 37,154 nuclei comprising 14 cell types in vastus lateralis samples collected before and 3.5 h after either 40 min cycling exercise at 70% VO₂max or 40 min supine rest. Both shared and cell-type-specific regulatory programs were identified. Differential gene expression and accessibility sites are largely distinct within nuclei for each cell type a

SUBMITTER: Rubenstein AB 

PROVIDER: S-EPMC12212352 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature