Single-Cell RNA Sequencing of Rabbit Sclera at Different Developmental Stages: Unveiling Scleral Cells Atlas and the Heterogeneity of Fibroblasts.

ABSTRACT:

Purpose

This study aims to construct a single-cell transcriptomic atlas of the developing rabbit sclera to elucidate fibroblast heterogeneity, differentiation trajectories, matrisome expression patterns, and intercellular communication, while revealing conserved molecular features of scleral cells through cross-species analysis.

Methods

Single-cell RNA sequencing (scRNA-seq) was performed on scleral tissues from New Zealand rabbits at embryonic day 25 (E25) and postnatal days 7 (P7), 21 (P21), and 180 (P180). Libraries were prepared using the DNBelab C Series Kit and sequenced on the BGISEQ-2000 platform. Sequencing reads were aligned to the OryCun2.0 genome using STAR, and unique molecular identifier (UMI) count matrices were generated with PISA. Data preprocessing was cond

SUBMITTER: Li F 

PROVIDER: S-EPMC12212446 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature