Structural Variants of a Liver Fluke Derived Granulin Peptide Potently Stimulate Wound Healing.

ABSTRACT: Granulins are a family of growth factors involved in cell proliferation. The liver-fluke granulin, Ov-GRN-1, isolated from a carcinogenic liver fluke Opisthorchis viverrini, can significantly accelerate wound repair in vivo and in vitro. However, it is difficult to express Ov-GRN-1 in recombinant form at high yield, impeding its utility as a drug lead. Previously we reported that a truncated analogue ( Ov-GRN_{12-35_3s}) promotes healing of cutaneous wounds in mice. NMR analysis of this analogue indicates the presence of multiple conformations, most likely as a result of proline cis/ trans isomerization. To further investigate whether the proline residues are involved in adopting the multiple confirmations, we have synthesized analogues involving mutation of the proline residues. W

SUBMITTER: Dastpeyman M 

PROVIDER: S-EPMC12212974 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature