Density of T-cell Subsets in Colorectal Cancer in Relation to Disease-Specific Survival.

ABSTRACT:

Background

Prior studies have demonstrated that the overall density of T cells in colorectal tumors is favorably associated with colorectal cancer survival; however, few studies have considered the potentially distinct roles of heterogeneous T-cell subsets in different tissue regions in relation to colorectal cancer outcomes.

Methods

Including 1,113 colorectal cancer tumors from three observational studies, we conducted in situ T-cell profiling using a customized nine-plex [CD3, CD4, CD8, CD45RA, CD45RO, FOXP3, KRT (keratin), MKI67 (Ki-67), and DAPI] multispectral immunofluorescence assay. Multivariable-adjusted Cox proportional hazards models were used to estimate HRs and 95% confidence intervals for the associations of T-cell subset densities in both epithelial and stromal

SUBMITTER: Thomas CE 

PROVIDER: S-EPMC12213185 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature