Integrated miRNAome, transcriptome, and degradome analyses reveal the role of miRNA-mRNA modules in the biosynthesis of oridonin in <i>Isodon rubescens</i>.

ABSTRACT:

Introduction

Isodon rubescens contains many bioactive diterpenoids, especially oridonin, which are used both as medicines and drinks. However, the structure and content of the diterpenoids in I. rubescens vary greatly in response to different ecological environments. MicroRNAs (miRNAs) play a pivotal role in the biosynthesis of secondary metabolites; but their roles in I. rubescens are obscure.

Methods

This research involved conducting miRNAome, transcriptome, and degradome sequencing analysis of three ecotypes of I. rubescens. Furthermore, the regulation of two candidate miRNA-mRNA modules was validated through a dual-luciferase reporter system.

Results

In this study, a total of 1634 miRNAs were identified from 9 miRNAome libraries of thre

SUBMITTER: Zhu L 

PROVIDER: S-EPMC12213554 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature