Whole genome sequencing and functional annotation of <i>Fusarium oxysporum</i> f. sp. <i>lentis</i> to unravel virulence and secondary metabolite biosynthesis gene clusters.

ABSTRACT:

Background

Fusarium oxysporum f. sp. lentis is a major fungal pathogen that causes vascular wilt in lentil crops, leading to significant reductions in yield. Despite its importance, the genetic underpinnings of this pathogen remain poorly understood.

Methods

We performed whole-genome sequencing of F. oxysporum f. sp. lentis using the Illumina Shotgun Sequencing platform. The resulting high-quality genome assembly consisted of 12,366 contigs with a total length of 124.48 Mb. Genome completeness was evaluated using Benchmarking Universal Single-Copy Orthologs (BUSCO) analysis, and functional annotation was performed through comparisons with several public databases, including Uniprot, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG

SUBMITTER: Kharte S 

PROVIDER: S-EPMC12213878 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature