Electroosmotic Flow-Based Nanoinjection Technique Using a Nanopipette for Green Microalgae.

ABSTRACT: Microalgae are gaining attention as promising candidates for CO₂ fixation and biomaterial production due to their non-competition with food and feed resources, as well as their high growth rates and photosynthetic efficiency. To expand their application as hosts for biomaterial production, it is essential to develop efficient and versatile gene modification technologies for microalgal metabolic engineering. Achieving this requires the establishment of an effective and broadly applicable material delivery method across diverse microalgal species. In this study, we developed a novel nanoinjection technique for single microalgal cells, utilizing electroosmotic flow through a nano-sized pipette (nanopipette). This nanopipette enables precise, automated delivery of solutions into cells at the f

SUBMITTER: Tanaka T 

PROVIDER: S-EPMC12213965 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature