Oxygen attachment dissociation (OAD) MS/MS in the identification of positional isomers of dysregulated lipids detected in an ethanol exposure metabolomics study in mice.

ABSTRACT:

Introduction

In metabolic profiling studies the structural characterisation of lipids requires the identification of the head group, carbon number and the position(s) of carbon-carbon double bonds (C = C). Locating the position of double bonds is vital since minor structural differences between positional isomers can alter a lipid's biochemical function.

Objectives

Oxygen Attachment Dissociation (OAD) is a novel fragmentation technology that enables the localisation of C = C double bonds in lipids. To evaluate its use in the structural characterisation of lipids, OAD has been applied in a discovery-based untargeted analysis of the metabolic impact of acute ethanol exposure in a mouse model.

Methods

UHPLC-OAD-MS/MS was used to enhance the identification of lipids found

SUBMITTER: Armitage EG 

PROVIDER: S-EPMC12213981 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature