Manipulating DNA and RNA structures via click-to-release caged nucleic acids for biological and biomedical applications.

ABSTRACT: Effectively controlling the structures of DNA and RNA is crucial for their functional utilization in material development, biological regulation, and medical applications. Here, we present a gain-of-function strategy for controlling DNA and RNA structures using an inverse electron-demand Diels-Alder (IEDDA) based click-to-release reaction. By incorporating click reaction-cleavable caged moiety into oligonucleotides, we disrupt activated base pairs, allowing controlled release of biofunctional higher-order nucleic acid structures. This click-to-release caged DNA was employed to control DNA duplex formation. Next, we demonstrated the utility of "click-to-release" strategy for regulated release of Z-DNA or Z-RNA and bind associated proteins. In addition, the approach was used to manipulated G

SUBMITTER: Wang S 

PROVIDER: S-EPMC12214008 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature