Differential oligomerization regulates PHF13 chromatin affinity and function.

ABSTRACT: PHF13 is a H3K4me3 epigenetic reader that modulates key chromatin processes including transcription, DNA damage response, and chromatin architecture. PHF13 is found aberrantly regulated in different cancers and its misexpression alters the epigenetic landscape of key transcription factors that regulate epithelial-to-mesenchymal transition. In this study, we sought to understand how PHF13's chromatin affinity and diverse chromatin functions are intrinsically regulated. Our results show that PHF13 can oligomerize via conserved ordered regions in its N- and C- terminus increasing its chromatin valence and avidity, promoting polymer-polymer phase separation (PPPS) and chromatin inaccessibility. Impressively, a ∼3- to 5-fold overexpression of PHF13 was sufficient to globally compact chromatin v

SUBMITTER: Rossi F 

PROVIDER: S-EPMC12214034 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature