MicroRNA-1 regulates metabolic flexibility by programming adult skeletal muscle pyruvate metabolism.

ABSTRACT:

Objective

Metabolic flexibility refers to the ability of tissues to adjust cellular fuel choice in response to conditional changes in metabolic demand and activity. A loss of metabolic flexibility is a defining feature of various diseases and cellular dysfunction. This study investigated the role of microRNA-1 (miR-1), the most abundant microRNA in skeletal muscle, in maintaining whole-body metabolic flexibility.

Methods

We used an inducible, skeletal muscle-specific knockout (KO) mouse model to examine miR-1 function. Argonaute 2 enhanced crosslinking and immunoprecipitation sequencing (AGO2 eCLIP-seq) and RNA-seq analyses identified miR-1 target genes. Metabolism was investigated using metabolomics, proteomics, and comprehensive bioenergetic and activity phenotyping. Corro

SUBMITTER: Ismaeel A 

PROVIDER: S-EPMC12214129 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature