Rapid DNA and RNA isolation from few or single cells using low-cost NAxtra magnetic nanoparticles.

ABSTRACT: A novel, cost-effective nucleic acid (NA) isolation method for purifying total NA, DNA, or RNA from both two- and three-dimensional cell cultures has been developed at the Norwegian University of Science and Technology utilizing NAxtra magnetic nanoparticles. This method achieves comparable yields to existing isolation kits while offering significant improvements in cost and processing speed. However, the original protocol was not optimized for small-cell numbers or single-cell applications. Given the growing interest in single-cell and rare-cell population studies, there is a critical need for more sensitive isolation techniques. In this study, we have enhanced the sensitivity of the NAxtra-based isolation method to facilitate mid- to high-throughput purification from as few as 10,000 cel

SUBMITTER: Starheim EJ 

PROVIDER: S-EPMC12214658 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature