Nanoscale structural alteration of lung collagen in response to strain and bleomycin injury.

ABSTRACT: The link between the structural organization of the fibrillar components of lung extracellular matrix (ECM), local tissue stiffness and global viscoelastic behaviour is not known. Here we investigated the effect of injurious mechanical ventilation on the local lung tissue stiffness using 4D synchrotron phase-contrast micro-CT, in normal lung and 7 days after intratracheal bleomycin induced lung injury in anesthetized rats. Quantitative maps of local lung strain (ε) were computed within aerated lung acini, using a stepwise image registration method. Fibrillar organization of collagen and elastin at the nanoscale was measured using synchrotron small-angle x-ray scattering (SAXS). Local microscopic tissue ε was reduced in the aerated acini of normal lungs post injurious ventilation and in ble

SUBMITTER: Deyhle RT 

PROVIDER: S-EPMC12214749 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature