Biosourcing and optimization of fungal lipase production from cheap agro waste via solid state fermentation.

ABSTRACT: The quest for economically viable sources of microbial lipases has been a primary focus for microbial enzymologists. This research aimed to screen, identify, and produce lipase from fungi using inexpensive agro-wastes as substrates. Samples of palm oil mill effluent were screened for lipolytic fungi using solid agar and submerged fermentation. Morphological and molecular methods were employed for isolate identification. Lipase activity was measured via spectrophotometric assays with p-nitrophenyl palmitate (p-NPP) as the substrate. The effect of medium composition on lipase production, the mineral and proximate compositions of the agro waste used as carbon sources via solid state fermentation were evaluated, followed by lipase purification and kinetic studies. Out of the 25 fungi screened,

SUBMITTER: Adebami GE 

PROVIDER: S-EPMC12214756 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature