ZFHX3 is integral to androgen/AR signaling involving protein association with AR in prostate cancer cells.

ABSTRACT: The androgen receptor (AR) signaling drives prostatic development and carcinogenesis, whereas the zinc finger homeobox 3 (ZFHX3) transcription factor modulates these processes. AR upregulates ZFHX3 transcription, but whether and how ZFHX3 plays a role in the AR signaling is unknown. RNA-seq was used to identify AR target genes that were also affected by ZFHX3 loss. Gene expression changes were verified using western blotting and qPCR. Immunoprecipitation, luciferase promoter-reporter assay, and western blotting were performed to assess ZFHX3's impact on AR transcriptional activity and ZFHX3 and AR protein interaction. Cell proliferation and colony formation assays were used to evaluate ZFHX3's impact on AR function. Kaplan-Meier analysis assessed the association of AR/ZFHX3 expression with

SUBMITTER: Fu X 

PROVIDER: S-EPMC12214913 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature