Dynamics and genetic variation of dengue virus serotypes circulating during the 2022 outbreak in Karachi.

ABSTRACT: Dengue virus (DENV) poses a critical health threat causing millions of cases every year. The absence of proof-reading activity of the RNA-dependent RNA polymerases (RdRp) leads to significant mutations in its genome. This may result in genetic diversity among the genotypes which contribute to severe complications during the infection. Targeting these mutations may help to understand the viral transmission and identify a potential treatment. An aggregate of 152 dengue positive serum samples were collected from different tertiary care hospitals in Karachi. RNA isolation, cDNA synthesis as well as amplification of the C-prM region was performed, followed by the Sanger sequencing. The resultant sequences of each serotype were aligned with their respective genome sequences and phylogenetic tree

SUBMITTER: Tariq F 

PROVIDER: S-EPMC12215615 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature