A new nuclear marker for quantitative analysis of wild boar and domestic pig meat in game meat products using PLAG1 zinc finger gene.

ABSTRACT: Wild boar meat and pork are commonly added to game products, either as forefront or background ingredients. Due to their lower cost and wider availability compared to deer species, there is a potential for producers to misrepresent the inclusion of these species in more expensive game products. Therefore, a novel, highly specific TaqMan qPCR method targeting a single-copy 127-bp region of the PLAG1 zinc finger gene was developed for quantifying these subspecies in processed foodstuff. The individual qPCR systems, adapted to different processing conditions (raw and thermally treated), were implemented using a matrix-adapted reference system. These systems were validated using laboratory-prepared wild boar-in-beef patties. The assay achieved satisfactory performance parameters for fraud dete

SUBMITTER: Adenuga BM 

PROVIDER: S-EPMC12216201 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature