Rapid detection of antibiotic resistance in Burkholderia pseudomallei using MALDI-TOF mass spectrometry.

ABSTRACT: Antibiotic resistance in Burkholderia pseudomallei (Bp) is a growing public health concern requiring urgent attention. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as a rapid bacterial identification tool with potential for future diagnostic applications. This study investigates differences in MALDI-TOF spectra of antibiotic-resistant Bp strains from northeastern Thailand without any pretreatment steps. A total of 300 Bp isolates were tested for minimum inhibitory concentration (MIC) values against five antibiotics: ceftazidime, meropenem, imipenem, co-amoxiclav, and co-trimoxazole. MALDI-TOF spectra were obtained using the direct colony method and analysed for correlations with antibiotic resistance patterns. Thirteen isolates exh

SUBMITTER: Nithimongkolchai N 

PROVIDER: S-EPMC12216213 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature