The mechanism of Bletilla striata inhibiting glioma proliferation through the PI3K/AKT/mTOR signaling pathway based on network Pharmacology analysis.

ABSTRACT: This study aims to elucidate the potential mechanisms underlying the therapeutic effects of Bletilla striata in glioma. Targets of Bletilla striata and glioma were predicted using TCMSP, SwissTargetPrediction, GeneCards, and other databases. A "drug-ingredient-target" network and protein-protein interaction (PPI) network were constructed, with core targets identified via topological analysis. Functional enrichment (GO/KEGG, DAVID), molecular docking, and experimental validations (MTT, scratch assay, RT-PCR, Western blot) were performed. A total of 11 active ingredients of Bletilla striata, 456 corresponding targets, and 2,830 glioma-related targets were identified. Nine core targets (AKT1, STAT3, mTOR, etc.) were identified. GO analysis indicated that these targets were primarily involved

SUBMITTER: Ren F 

PROVIDER: S-EPMC12216624 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature