Crystal structure of coagulation factor XII N-terminal domains 1-5.

ABSTRACT: Factor XIIa (FXIIa) is generated from its zymogen factor XII (FXII) by contact with polyanions such as inorganic polyphosphates. FXIIa cleaves the substrates prekallikrein and factor XI, triggering inflammatory cascades and plasma coagulation. From the N-terminus, FXII has fibronectin type II (FnII), epidermal growth factor-1 (EGF1), fibronectin type I (FnI), EGF2 and kringle domains. The N-terminal domains of FXII mediate polyanion and Zn²⁺ binding. To understand how ligand binding to polyanions and Zn²⁺ is coordinated across multiple domains, we determined the crystal structure of recombinant FXII domains 1-5 (FXII^HC5) to 3.4 Å resolution. A separate crystal structure of the isolated FXII FnII domain at 1.2 Å resolution revealed two bound Zn²⁺

SUBMITTER: Saleem M 

PROVIDER: S-EPMC12216678 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature