E3 ligase TRIM22 promotes melanoma proliferation by regulating cell cycle progression through K63-linked ubiquitination of p21.

ABSTRACT: Melanoma, a highly aggressive skin cancer with limited therapeutic options, demonstrates poor prognosis in advanced stages. Tripartite motif-containing 22 (TRIM22), an E3 ubiquitin ligase of the tripartite motif (TRIM) family, is implicated in tumorigenesis, but its working mechanism remains poorly understood in melanoma. In this study, we found that expression of TRIM22 was abnormally upregulated in melanoma tissues, correlating with tumor stages. Functional analysis demonstrated that TRIM22 promoted melanoma cell proliferation in vitro. Furthermore, we found that in malignant melanoma, TRIM22 expression is negatively correlated to the level of p21, an inhibitor of cell cycle. With quantitative real-time PCR (qRT-PCR) assay and cycloheximide (CHX) treatment, we confirmed that TRIM22 suppr

SUBMITTER: Jin CX 

PROVIDER: S-EPMC12216916 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature