Evaluation of the potential for diagnosis of fungal keratitis using a Fusarium-specific antibody.

ABSTRACT: The increasing number of contact lens users correlates with a rise in the incidence of fungal keratitis. Fungal keratitis can lead to blindness if not treated promptly, and its early symptoms are similar to those of bacterial and amoebic keratitis, making rapid diagnosis challenging. This study aimed to assess the potential of using a peptide antibody against the fungal-specific protein ERG24, which encodes sterol C-14 reductase, to differentiate fungal keratitis from other forms of keratitis. The specificity of the ERG24 antibody was assessed through Western blot and enzyme-linked immunosorbent assay (ELISA). Immunocytochemistry (ICC) was performed by co-culturing two types of fungi, Acanthamoeba, and two bacterial strains with human corneal epithelial (HCE) cells. Additionally, to evalua

SUBMITTER: Jo HJ 

PROVIDER: S-EPMC12217492 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature