TIMP1 promotes microglia M2 polarization through MAPK pathway to ameliorate early brain injury after ischemia.

ABSTRACT:

Objective

To explore the molecular regulatory mechanisms and biomarkers in regulating early brain injury (EBI) and inflammatory response after ischemic stroke (IS).

Methods

Gene expression profiles of GSE148350, GSE35338, and GSE58294 were analyzed to screen the core genes involved in EBI after IS. Middle cerebral artery occlusion and reperfusion (MCAO/R) model and oxygen and glucose deprivation and reoxygenation (OGD/R) model were used to construct in vivo and in vitro IS models. In MCAO/R model, the effects of tissue inhibitor of metalloproteinase-1 (TIMP1) were investigated by Zea longa score, brain water content assessment and histological analysis. In OGD/R model, after TIMP1 was overexpressed in BV2 cells, M1 and M2 polarization markers (iNOS and Arg-1) in BV2 cells we

SUBMITTER: Zhao K 

PROVIDER: S-EPMC12217830 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature