An engineered self-cleavage fusion system for the production of chimaera spider silk proteins.

ABSTRACT:

Background

Spidroins are well-known for their exceptional mechanical properties, which have inspired extensive research and applications across various fields. Large-scale production of spidroin continues to face major challenges due to the complexity involved in inducing host organisms to express full-length spidroins. This process requires advanced techniques and has issues such as plasmid instability and potential misfolding.

Results

In this study, we developed a novel expression system by combining a fusion tag with a self-cleavage intein, enabling the convenient expression of three chimeric spidroins with varying numbers of repetitive units. After optimising expression conditions, NT2RepCT, NT4RepCT and NT6RepCT spidroins were obtained in soluble form, with yields of 26

SUBMITTER: Su Y 

PROVIDER: S-EPMC12218096 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature