Lactulose selectively stimulates members of the gut microbiota, as determined by multi-modal activity-based sorting.

ABSTRACT: There is much interest in the development of dietary supplements that selectively promote the growth of beneficial gut bacteria. The selectivity of many candidate prebiotics has, however, not been thoroughly investigated. Here, we evaluated stimulation of the human gut microbiota by the disaccharide lactulose using an ex vivo multimodal activity-based cell sorting approach. Incubation of human donor stool with lactulose resulted in growth or stimulation of a restricted diversity of bacterial genera, most prominently Bifidobacterium, Collinsella, and Lactococcus. Physiological analysis of lactulose-responsive strains isolated by Raman activated cell sorting revealed that most were capable of lactulose degradation. Among these isolates, Lactococcus lactis c

SUBMITTER: Rasoulimehrabani H 

PROVIDER: S-EPMC12218441 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature