Development of a candidate mRNA vaccine based on Multi-Peptide targeting VP4 of rotavirus A: an immunoinformatics and molecular dynamics approach.

ABSTRACT: Rotavirus (RV) is a common double-stranded RNA virus that causes diarrheal disease in young children. The prevalent species, Rotavirus A (RVA), is responsible for over 90% of human RV infections. With significant morbidity and mortality, this pathogen poses a serious global health challenge, particularly in underdeveloped countries. This study presents an immunoinformatics approach for designing an mRNA vaccine based on a multi-peptide construct to elicit robust immune responses against RVA. The VP4 was analyzed from 40 sequences using phylogenetic analysis. Prediction of cytotoxic (CTL) and helper T cell (HTL) epitopes was performed and validated. The 17 high-conservancy CTL/HTL epitopes were selected for vaccine construction. The mRNA vaccine based on multi-peptide was engineered with hu

SUBMITTER: Aram C 

PROVIDER: S-EPMC12218802 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature