Dominant and cross-reactive S1-specific memory B cell response induced by primary SARS-CoV-2 infection.

ABSTRACT: The protective component of specific memory B cells (MBCs) response relative to serum antibody response in primary SARS-CoV-2 infection is not well understood. Using a relatively unbiased B-cell culture method with a limited number of MBCs in each well (100 cells/well), we characterized the fine specificity of MBC responses against SARS-CoV-2 infection. While serum spike antibody is predominantly against S2 domain, the memory B cells mainly recognize S1 domain. The 44.4-85.3% of S-binding MBCs are specific to S1 domain. High frequency of MBCs (30-62% of SARS-CoV-2 S-specific MBCs) cross-reacting with SARS-CoV S has also been demonstrated. 22-33% of S1-binding MBCs were cross-reactive with the SARS-CoV RBD. In addition, a panel of human monoclonal Ab was derived from S1-binding MBCs recogni

SUBMITTER: Xing X 

PROVIDER: S-EPMC12218836 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature