Identification of pyroptosis related genes and subtypes in atherosclerosis using multiomic and single cell analysis.

ABSTRACT: Atherosclerosis (AS), the leading cause of cardiovascular diseases, is a chronic inflammatory disorder involving lipid metabolism, immune dysregulation, and cell death. Pyroptosis, a form of inflammatory programmed cell death, is implicated in AS progression, yet its molecular mechanisms and therapeutic potential remain incompletely understood. A multi-omics framework integrating transcriptomics, single-cell RNA sequencing, and machine learning to identify and prioritize pyroptosis-related genes (PRGs) in AS. Functional enrichment, immune infiltration profiling, and protein-protein interaction network analyses were conducted. Experimental validation was conducted using in vitro and in vivo models. Thirty-six PRGs were identified, with TREM2, TNF, MMP9, IL1B, and CASP1 emerging as key regul

SUBMITTER: Jiang K 

PROVIDER: S-EPMC12218848 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature