Glycoproteomic profiling of serum-derived small extracellular vesicles enriched via ultracentrifugation and affinity-based techniques.

ABSTRACT: Small extracellular vesicles (sEVs) are gaining recognition as potential biomarkers for diseases, including cancer, due to their involvement in key pathophysiological processes. However, the glycosylation of EVs and the specific roles of their glycans remain poorly understood. While several methods exist for isolating sEVs from complex biological samples, achieving sufficient purity and quantity for mass spectrometry-based glycoproteomic analysis remains a significant challenge. In this study, we compared two commonly used isolation methods, ultracentrifugation (UC) and immunoaffinity capture (MagCapture kit), across different starting volumes of human serum (200 µL and 500 µL) to evaluate their performance for downstream glycoproteomic analysis. While prior studies have examined protein c

SUBMITTER: Fowowe M 

PROVIDER: S-EPMC12218995 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature